|
|
Gut microbiome and its changes related to therapy of chronic diseases
Hurych, Jakub ; Cinek, Ondřej (advisor) ; Kolář, Milan (referee) ; Falt, Přemysl (referee)
This work examines the response of the gut microbiome to therapeutic interventions in three longitudinal studies of chronic gastrointestinal diseases: Crohn's disease, celiac autoimmunity and irritable bowel syndrome. Multiple methods of stool microbiome analysis (especially massively parallel 16S rDNA or 18S rDNA sequencing and metagenomic sequencing) followed by bioinformatic and statistical analysis were used. In Crohn's disease, we detected a previously undescribed secondary nature of changes in the gut bacteriome after anti-TNF treatment. In celiac disease autoimmunity, where previous works described an effect of probiotic intervention on serological markers of the disease, the gut bacteriome and metabolome, we described the absence of significant changes in beneficial gut protozoa. In irritable bowel syndrome, we observed a significant response of the bacteriome after administering four doses of mixed microbiota transplantation but no response in the reduction of clinical symptoms. The results of these studies could contribute to a better understanding of the gut microbiome's role in the pathogenesis of these serious diseases. Keywords: microbiome, Crohn's disease, celiac disease, irritable bowel syndrome
|
|
|
Výskyt laktózové intolerance v české populaci
CHÁNOVÁ, Jiřina
The aim of this diploma thesis is to summarize the current knowledge on the issue of very common gastrointestinal disorder - lactose intolerance. In the experimental part, the occurrence of genotypic frequencies in the MCM6 gene was screened. Specifically, the occurrence of two single nucleotide polymorphisms C/T-13910 and G/A-22018, which are associated with primary hypolactasia. A further aim of the work was to evaluate the possible association between lactose intolerance and irritable bowel syndrome.
|
|
|
Investigation of food intolerances using Immunolab IgG4 Screen Nutritional Lineblot.
KARÁSKOVÁ, Pavla
Food intolerance is defined as a response of G immunoglobulins (IgG) to antigens, which come from specific types of food. Statistical analyses show that 45 - 60% of population suffer from intolerance to at least one type of foodstuff that may lead to clinical symptoms or to worsen such symptoms. Majority of people is not aware of their intolerance and therefore various symptoms are unnecessarily and unsuccessfully treated by medication. Signs of an intolerance can vary from skin irritation, digestive problems, up to overweight. The aim of my bachelor thesis was to prepare a professional literature review. Then to master practical methods enabling detection of 20 food intolerances from blood plasma using Immunolab IgG4 Screen Nutritional Lineblot, and presentation of findings about food intolerances. Theoretical part of the thesis is focused on differences between a food intolerance and food allergy, the most common causes of intolerances and their possible association with other diseases. Ways of investigations of intolerances are analysed together with a summary of experts' positions over this testing. Practical part of the thesis, which I performed in genetic laboratories GENLABS in České Budějovice, contains a precise description of performed method used for a detection of food intolerances and statistical analysis of retained results.
|
guest ::
